Open AccessContamination of street food with multidrug-resistant Salmonella, in Ouagadougou, Burkina Faso
Author(s) -
Marguerite E. M. Nikiema,
María Pardos de la Gándara,
Kiswensida A. M. Compaore,
Absétou Ky Ba,
Karna D. Soro,
Philippe Augustin NIKIEMA,
Nicolas Barro,
Lassana Sangaré,
FrançoisXavier Weill
Publication year - 2021
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0253312
Subject(s) - salmonella , salmonella enterica , serotype , biology , multiple drug resistance , food contaminant , antibiotic resistance , salmonella infection , veterinary medicine , microbiology and biotechnology , drug resistance , medicine , antibiotics , food science , genetics , bacteria
Background Gastrointestinal infections are a global public health problem. In Burkina Faso, West Africa, exposure to Salmonella through the consumption of unhygienic street food represents a major risk of infection requiring detailed evaluation. Methods Between June 2017 and July 2018, we sampled 201 street food stalls, in 11 geographic sectors of Ouagadougou, Burkina Faso. We checked for Salmonella contamination in 201 sandwiches (one per seller), according to the ISO 6579:2002 standard. All Salmonella isolates were characterized by serotyping and antimicrobial susceptibility testing, and whole-genome sequencing was performed on a subset of isolates, to investigate their phylogenetic relationships and antimicrobial resistance determinants. Results The prevalence of Salmonella enterica was 17.9% (36/201) and the Salmonella isolates belonged to 16 different serotypes, the most frequent being Kentucky, Derby and Tennessee, with five isolates each. Six Salmonella isolates from serotypes Brancaster and Kentucky were multidrug-resistant (MDR). Whole-genome sequencing revealed that four of these MDR isolates belonged to the emergent S . enterica serotype Kentucky clone ST198-X1 and to an invasive lineage of S . enterica serotype Enteritidis (West African clade). Conclusion This study reveals a high prevalence of Salmonella spp. in sandwiches sold in Ouagadougou. The presence of MDR Salmonella in food on sale detected in this study is also matter of concern.