Open AccessIn-vitro model for bacterial growth inhibition of compartmentalized infection treated by an ultra-high concentration of antibiotics
Author(s) -
Moris Topaz,
Abed Athamna,
Itamar Ashkenazi,
Baruch Shpitz,
Sarit Freimann
Publication year - 2021
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0252724
Subject(s) - antibiotics , pseudomonas aeruginosa , microbiology and biotechnology , staphylococcus aureus , gentamicin , amikacin , bacterial growth , escherichia coli , biology , minimum inhibitory concentration , in vitro , bacteria , chemistry , biochemistry , gene , genetics
Background Pseudomonas aeruginosa (P . aeruginosa) , Escherichia coli (E . coli) , and Staphylococcus aureus (S . aureus) are common pathogens encountered in infected cardiovascular-implantable electronic device (CIED). Continuous, in-situ targeted, ultra-high concentration antibiotic (CITA) treatment is a novel antibiotic treatment approach for localized infections. CITA provides sufficient local antibiotic concentrations to heavily infected cavities while avoiding systemic toxicity. Aim In-vitro confirmation of the efficacy of the CITA treatment approach in simulated compartmentalized infections. Materials and methods A rapid automated bacterial culture analyzing system) Uro4 HB&L ™ (was applied to compare the efficacy of selected antibiotics at a standard minimal inhibitory concentration (1MIC), 4MIC, and CITA at 10 3 MIC, for growth inhibition of high bacterial loads (10 6 colony-forming-units/ml) of ATCC strains of P . aeruginosa , E . coli , and S . aureus . Results The addition of gentamicin and amikacin at 1MIC concentrations only temporarily inhibited the exponential growth of E . coli and P . aeruginosa . 4MIC level extended the delay of exponential bacterial growth. Increasing concentrations of vancomycin similarly temporarily delayed S . aureus growth. All tested antibiotics at CITA of 10 3 MIC totally inhibited the exponential growth of the tested bacteria through 72 hours of exposure. (P<0.001). Conclusion In this in-vitro model, CITA at 10 3 MIC effectively inhibited exponential bacterial growth of high loads of P . aeruginosa , E . coli , and S . aureus . This model offers preliminary laboratory support for the benefit of the in-situ antibiotic treatment, providing ultra-high concentrations directly at the compartmentalized infection site, not achievable by the conventional intravenous and oral routes.