Open AccessNovel low shear 3D bioreactor for high purity mesenchymal stem cell production
Author(s) -
Alan R. Burns,
Corinna Doris,
Kevin Vehar,
Vinit Saxena,
Cameron Bardliving,
P. Ayazi Shamlou,
M. Ian Phillips
Publication year - 2021
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0252575
Subject(s) - mesenchymal stem cell , bioreactor , stem cell , regenerative medicine , cell culture , biomedical engineering , tissue engineering , matrix (chemical analysis) , materials science , polylactic acid , microbiology and biotechnology , cell growth , chemistry , biology , medicine , biochemistry , composite material , genetics , organic chemistry , polymer
Bone marrow derived human Mesenchymal Stem Cells (hMSCs) are an attractive candidate for regenerative medicine. However, their harvest can be invasive, painful, and expensive, making it difficult to supply the enormous amount of pure hMSCs needed for future allogeneic therapies. Because of this, a robust method of scaled bioreactor culture must be designed to supply the need for high purity, high density hMSC yields. Here we test a scaled down model of a novel bioreactor consisting of an unsubmerged 3D printed Polylactic Acid (PLA) lattice matrix wetted by culture media. The growth matrix is uniform, replicable, and biocompatible, enabling homogenous cell culture in three dimensions. The goal of this study was to prove that hMSCs would culture well in this novel bioreactor design. The system tested resulted in comparable stem cell yields to other cell culture systems using bone marrow derived hMSCs, while maintaining viability (96.54% ±2.82), high purity (>98% expression of combined positive markers), and differentiation potential.