Open AccessPseudoprevotella muciniphila gen. nov., sp. nov., a mucin-degrading bacterium attached to the bovine rumen epithelium
Author(s) -
Sang Weon Na,
Byung Hee Chun,
Seok-Hyeon Beak,
Shehzad Abid Khan,
Md. Najmul Haque,
Jae Sung Lee,
Che Ok Jeon,
Sang-Suk Lee,
Myunggi Baik
Publication year - 2021
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0251791
Subject(s) - biology , mucin , microbiology and biotechnology , bacteria , strain (injury) , rumen , akkermansia muciniphila , virulence , biochemistry , fermentation , gene , genetics , anatomy
A Gram-negative, strictly anaerobic mucin-degrading bacterium, which we designated strain E39 T , was isolated from the rumen epithelium of Korean cattle. The cells were non-motile and had a coccus morphology. Growth of strain E39 T was observed at 30–45°C (optimum, 39°C), pH 6.5–8.5 (optimum, pH 7.5), and in the presence of 0.0–1.0% (w/v) NaCl (optimum, 0.0–0.5%). Strain E39 T contained C 16:0 , C 18:0 , C 18:1 ω 9 c , iso-C 15:0 , and anteiso-C 15:0 as the major fatty acids. The major polar lipids were phosphatidylethanolamine, unidentified aminophospholipid, and unidentified lipids. The major respiratory isoprenoid quinones were MK-8 and MK-9. The major fermented end-products of mucin were acetate and succinate. The G+C content of the genomic DNA was 46.4 mol%. Strain E39 T was most closely related to Alloprevotella rava 81/4-12 T with an 87.3% 16S rRNA gene sequence similarity. On the basis of phenotypic, chemotaxonomic, and molecular properties, strain E39 T represents a novel genus of the family Prevotellaceae ; as such, the name Pseudoprevotella muciniphila gen. nov., sp. nov. is proposed. A functional annotation of the whole genome sequences of P . muciniphila E39 T revealed that this bacterium has a putative mucin-degrading pathway and biosynthetic pathways of extracellular polymeric substances and virulence factors which enable bacteria to adhere to the epithelial cells and avoid the host’s immune responses.