Open AccessEffects of osmotic pressure on the irreversible electroporation in giant lipid vesicles
Author(s) -
Malay Kumar Sarkar,
Mohammad Abu Sayem Karal,
Marzuk Ahmed,
Md. Kabir Ahamed,
Shareef Ahammed,
Sabrina Sharmin,
Sayed Ul Alam Shibly
Publication year - 2021
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0251690
Subject(s) - vesicle , osmotic pressure , membrane , chemistry , biophysics , irreversible electroporation , electroporation , membrane potential , tension (geology) , swelling , chromatography , biochemistry , materials science , biology , ultimate tensile strength , gene , metallurgy , composite material
Irreversible electroporation (IRE) is a nonthermal tumor/cell ablation technique in which a series of high-voltage short pulses are used. As a new approach, we aimed to investigate the rupture of giant unilamellar vesicles (GUVs) using the IRE technique under different osmotic pressures (Π), and estimated the membrane tension due to Π. Two categories of GUVs were used in this study. One was prepared with a mixture of dioleoylphosphatidylglycerol (DOPG), dioleoylphosphatidylcholine (DOPC) and cholesterol (chol) for obtaining more biological relevance while other with a mixture of DOPG and DOPC, with specific molar ratios. We determined the rate constant ( k p ) of rupture of DOPG/DOPC/chol (46/39/15)-GUVs and DOPG/DOPC (40/60)-GUVs induced by constant electric tension ( σ c ) under different Π. The σ c dependent k p values were fitted with a theoretical equation, and the corresponding membrane tension ( σ oseq ) at swelling equilibrium under Π was estimated. The estimated membrane tension agreed well with the theoretical calculation within the experimental error. Interestingly, the values of σ oseq were almost same for both types of synthesized GUVs under same osmotic pressure. We also examined the sucrose leakage, due to large osmotic pressure-induced pore formation, from the inside of DOPG/DOPC/chol(46/39/15)-GUVs. The estimated membrane tension due to large Π at which sucrose leaked out was very similar to the electric tension at which GUVs were ruptured without Π. We explained the σ c and Π induced pore formation in the lipid membranes of GUVs.