Open AccessUV light-emitting diode (UV-LED) at 265 nm as a potential light source for disinfecting human platelet concentrates
Author(s) -
Tetsusuke Hayashi,
Kumiko Oguma,
Yoshihiro Fujimura,
Rie Furuta,
Mitsunobu Tanaka,
Mikako Masaki,
Yasuhito Shinbata,
Takafumi Kimura,
Yoshihiko Tani,
Fumiya Hirayama,
Yoshihiro Takihara,
Koki Takahashi
Publication year - 2021
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0251650
Subject(s) - platelet , bacillus cereus , chemistry , platelet transfusion , staphylococcus aureus , biophysics , platelet activation , platelet rich plasma , irradiation , sepsis , escherichia coli , fluence , bacteria , medicine , biochemistry , surgery , biology , immunology , ion , genetics , physics , organic chemistry , nuclear physics , gene
The risk of sepsis through bacterial transmission is one of the most serious problems in platelet transfusion. In processing platelet concentrates (PCs), several methods have been put into practice to minimize the risk of bacterial transmission, such as stringent monitoring by cultivation assays and inactivation treatment by photoirradiation with or without chemical agents. As another potential option, we applied a light-emitting diode (LED) with a peak emission wavelength of 265 nm, which has been shown to be effective for water, to disinfect PCs. In a bench-scale UV-LED exposure setup, a 10-min irradiation, corresponding to an average fluence of 9.2 mJ/cm 2 , resulted in >2.0 log, 1.0 log, and 0.6 log inactivation (mean, n = 6) of Escherichia coli , Staphylococcus aureus , and Bacillus cereus , respectively, in non-diluted plasma PCs. After a 30-min exposure, platelet counts decreased slightly (18 ± 7%: mean ± SD, n = 7); however, platelet surface expressions of CD42b, CD61, CD62P, and PAC-1 binding did not change significantly (P>0.005), and agonist-induced aggregation and adhesion/aggregation under flow conditions were well maintained. Our findings indicated that the 265 nm UV-LED has high potential as a novel disinfection method to ensure the microbial safety of platelet transfusion.