Open AccessAnalysis of blood parameters and molecular endometrial markers during early reperfusion in two ovine models of uterus transplantation
Author(s) -
Marie Carbonnel,
Nathalie Cornet,
Aurélie Revaux,
Angéline Favre-Inhofer,
Laurent Galio,
Mariam Raliou,
Anne CouturierTarrade,
Corinne Giraud-Delville,
Gilles Charpigny,
Valérie Gelin,
Olivier Dubois,
Barbara Hersant,
Romain Bosc,
Raphaël Coscas,
François Vialard,
Pascale ChavattePalmer,
Christophe Richard,
Olivier Sandra,
Jean Marc Ayoubi
Publication year - 2021
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0251474
Subject(s) - anastomosis , uterus , transplantation , andrology , medicine , endometrium , vein , in utero , surgery , urology , biology , pathology , pregnancy , fetus , genetics
The dissection of the veins is the trickiest step of Uterine transplantation (UTx). Performing the anastomosis of a single uterine vein could bring a therapeutic benefit and simplification of surgery and serve for managing unilateral venous thromboses. The objectives of this project were to evaluate the expression of early markers of ischemia-reperfusion and to compare findings following one or two vein anastomoses. Orthotopic uterine auto-transplantations were performed on an ovine model with anastomosis of either two (group 1) or one utero-ovarian veins (group 2). Blood gases, histology and ischemia- reperfusion markers transcripts ( PTGS2 , IL6 , IL8 , SOD2 , C3 , BAX/BCL2 and TLR4) were analyzed as well as PTGS2 protein expression using Western Blot and fluorescence immunolocalization on endometrial biopsies after 3h of reperfusion. Ten ewes were included in the experimentation, 4 were in group1, 3 in group 2, the others being sham operated controls. No significant differences were observed between the two phenotypes. Based on these results, the anastomosis of one single uterine vein appears to be an approach consistent with short-term graft survival. Further experiments will be needed to confirm the reliability of this approach, especially the long-term follow-up of the uterine graft including its ability to support gestation to term.