Open AccessCharacterization of genetically modified mice for phosphoglycerate mutase, a vitally-essential enzyme in glycolysis
Author(s) -
Takumi Mikawa,
Eri Shibata,
Midori Shimada,
Ken Ito,
Tomiko Ito,
Hiroaki Kanda,
Keiyo Takubo,
Atsuyoshi Shimada,
Matilde E. Lleonart,
Nobuya Inagaki,
Masayuki Yokode,
Hiroshi Kondoh
Publication year - 2021
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0250856
Subject(s) - phosphoglycerate mutase , glycolysis , biology , in vivo , knockout mouse , genetically modified mouse , enzyme , mutase , biochemistry , phosphoglycerate kinase , carbohydrate metabolism , transgene , genetics , gene
Glycolytic metabolism is closely involved in physiological homeostasis and pathophysiological states. Among glycolytic enzymes, phosphoglycerate mutase (PGAM) has been reported to exert certain physiological role in vitro , whereas its impact on glucose metabolism in vivo remains unclear. Here, we report the characterization of Pgam1 knockout mice. We observed that homozygous knockout mice of Pgam1 were embryonic lethal. Although we previously reported that both PGAM-1 and -2 affect global glycolytic profile of cancers in vitro , in vivo glucose parameters were less affected both in the heterozygous knockout of Pgam1 and in Pgam2 transgenic mice. Thus, the impact of PGAM on in vivo glucose metabolism is rather complex than expected before.