Open AccessAntiplatelet activity and chemical analysis of leaf and fruit extracts from Aristotelia chilensis
Author(s) -
Lyanne Rodríguez,
Andrés Trostchansky,
Irene Wood,
Mauricio Mastrogiovanni,
Hermine Vogel,
Benita González,
Mário Roberto Maróstica,
Eduardo Fuentes,
Iván Palomo
Publication year - 2021
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0250852
Subject(s) - anthocyanin , chemistry , food science , platelet , botany , platelet aggregation , biochemistry , platelet activation , biology , immunology
Aristotelia chilensis (Mol.) Stuntz, also known as maqui, is a plant native to Chile without chemical characterization and quantification of the bioactive compounds present in it. HPLC-UV and HPLC-MS/MS studies have shown the presence, at different concentrations, of phenolic and anthocyanin compounds in fruit and leave extracts of the domesticated maqui clones Luna Nueva, Morena, and Perla Negra. The extracts from leaves and unripe fruits of Luna Nueva and Morena clones significantly inhibit platelet aggregation induced by several agonists; the extracts inhibit platelet granule secretion by decreasing the exposure of P-selectin and CD63 at the platelet membrane. Reactive oxygen species formation in platelets is lower in the presence of maqui extracts. Statistical Pearson analysis supports the levels of phenolic and anthocyanin compounds being responsible for the antiaggregant maqui effects. This work is the first evidence of antiplatelet activity from Aristotelia chilensis giving added value to the use of leaves and unripe fruits from this species.