Open AccessHeat-inactivated Lactobacillus plantarum nF1 promotes intestinal health in Loperamide-induced constipation rats
Author(s) -
Seon-Ah Park,
Geum-Hwa Lee,
TheHiep Hoang,
Hwa Young Lee,
In-Yeong Kang,
Myong-Ja Chung,
Jong-Sik Jin,
Han-Jung Chae
Publication year - 2021
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0250354
Subject(s) - loperamide , constipation , lactobacillus plantarum , medicine , gastroenterology , gut flora , diarrhea , lactobacillus , immunology , biology , lactic acid , food science , fermentation , bacteria , genetics
Constipation is a common condition that affects individuals of all ages, and prolonged constipation needs to be prevented to avoid potential complications and reduce the additional stress on individuals with pre-medical conditions. This study aimed to evaluate the effects of heat-inactivated Lactobacillus plantarum (HLp-nF1) on loperamide-induced constipation in rats. Constipation-induced male rats were treated orally with low to high doses of HLp-nF1 and an anti-constipation medication Dulcolax for five weeks. Study has 8 groups, control group; loperamide-treated group; Dulcolax-treated group; treatment with 3.2 × 10 10 , 8 × 10 10 and 1.6 × 10 11 , cells/mL HLp-nF1; Loperamide + Dulcolax treated group. HLp-nF1 treated rats showed improvements in fecal pellet number, weight, water content, intestinal transit length, and contractility compared to the constipation-induced rats. Also, an increase in the intestine mucosal layer thickness and the number of mucin-producing crypt epithelial cells were observed in HLp-nF1-treated groups. Further, the levels of inflammatory cytokines levels were significantly downregulated by treatment with HLp-nF1 and Dulcolax. Notably, the metagenomics sequencing analysis demonstrated a similar genus pattern to the pre-preparation group and control with HLp-nF1 treatment. In conclusion, the administration of >3.2 × 10 10 cells/mL HLp-nF1 has a positive impact on the constipated rats overall health.