Open AccessA laboratory-based study to explore the use of honey-impregnated cards to detect chikungunya virus in mosquito saliva
Author(s) -
Lisa Fourniol,
Yoann Madec,
Laurence Mousson,
Marie Vazeille,
AnnaBella Failloux
Publication year - 2021
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0249471
Subject(s) - chikungunya , aedes albopictus , aedes aegypti , aedes , saliva , virology , virus , biology , mosquito control , arbovirus , dengue fever , malaria , immunology , ecology , larva , biochemistry
Mosquito control is implemented when arboviruses are detected in patients or in field-collected mosquitoes. However, mass screening of mosquitoes is usually laborious and expensive, requiring specialized expertise and equipment. Detection of virus in mosquito saliva using honey-impregnated filter papers seems to be a promising method as it is non-destructive and allows monitoring the viral excretion dynamics over time from the same mosquito. Here we test the use of filter papers to detect chikungunya virus in mosquito saliva in laboratory conditions, before proposing this method in large-scale mosquito surveillance programs. We found that 0.9 cm 2 cards impregnated with a 50% honey solution could replace the forced salivation technique as they offered a viral RNA detection until 7 days after oral infection of Aedes aegypti and Aedes albopictus mosquitoes with CHIKV.