Open AccessCan plasma antioxidants prevent DNA damage in oxidative stress condition induced by growth hormone deficiency? A pilot study
Author(s) -
Antonio Mancini,
Francesco Guidi,
Carmine Bruno,
Flavia Angelini,
Edoardo Vergani,
Paola Lanza,
Alvaro Mordente,
Elisabetta Meucci,
Andrea Silvestrini
Publication year - 2021
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0248971
Subject(s) - oxidative stress , medicine , dna damage , antioxidant , endocrinology , thymidine , oxidative phosphorylation , growth hormone deficiency , chemistry , hormone , growth hormone , biology , biochemistry , dna
Adult growth hormone deficiency (GHD), a condition characterized by increased oxidative stress, is related to augmented cardiovascular, metabolic and oncological risk. A case-control observational study has been performed to evaluate DNA oxidative damage analysing the production of thymidine-glycol in lymphocytes and its correlation with plasma antioxidant levels, evaluated as Total Antioxidant Capacity (TAC). GHD was diagnosed using GHRH 50μg iv+arginine 0,5 g/Kg test, with peak GH response <9 μg/L when BMI was <30 kg/m 2 or <4 μg/L when BMI was >30 kg/m 2 . Three groups were identified: total GHD (n = 16), partial GHD (n = 11), and controls (n = 12). Thymidine-glycol, TAC and IGF-1 have been determined respectively in lymphocytes, plasma and serum samples. When considering thymidine-glycol, we found a significant difference between total vs partial GHD and controls. Unexpectedly thymidine-glycol was lower in total GHD, also accompanied with a significant increase in plasmatic TAC. Our results showed that in adult GHD condition, the production of antioxidant species, in response to increased oxidative stress, could exert a protective effect on thymidine-glycol formation, and consequently on DNA intracellular damages. This pilot study could be inserted in the complex scenario of oxidative damage of GHD, a subtle, yet poorly defined condition, worthy of further insights.