Open AccessA cytofluorimetric analysis of a Saccharomyces cerevisiae population cultured in a fed-batch bioreactor
Author(s) -
Emanuela Palomba,
Valentina Tirelli,
Elisabetta de Alteriis,
Palma Parascandola,
C. Landi,
Stefano Mazzoleni,
Massimo Sanchez
Publication year - 2021
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0248382
Subject(s) - saccharomyces cerevisiae , yeast , population , bioreactor , biology , flow cytometry , biomass (ecology) , microbiology and biotechnology , biochemical engineering , computational biology , biochemistry , botany , genetics , engineering , demography , sociology , agronomy
The yeast Saccharomyces cerevisiae is a reference model system and one of the widely used microorganisms in many biotechnological processes. In industrial yeast applications, combined strategies aim to maximize biomass/product yield, with the fed-batch culture being one of the most frequently used. Flow cytometry (FCM) is widely applied in biotechnological processes and represents a key methodology to monitor cell population dynamics. We propose here an application of FCM in the analysis of yeast cell cycle along the time course of a typical S. cerevisiae fed-batch culture. We used two different dyes, SYTOX Green and SYBR Green, with the aim to better define each stage of cell cycle during S. cerevisiae fed-batch culture. The results provide novel insights in the use of FCM cell cycle analysis for the real-time monitoring of S . cerevisiae bioprocesses.