Open AccessFungal GH25 muramidases: New family members with applications in animal nutrition and a crystal structure at 0.78Å resolution
Author(s) -
Olga V. Moroz,
E.V. Blagova,
Edward J. Taylor,
J.P. Turkenburg,
L.K. Skov,
Garry P. Gippert,
Kirk Schnorr,
Ming Li,
Ye Liu,
Mikkel Klausen,
Marianne Thorup Cohn,
E. G. Schmidt,
Søren Nymand-Grarup,
G.J. Davies,
K.S. Wilson
Publication year - 2021
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0248190
Subject(s) - peptidoglycan , glycosidic bond , glycoside hydrolase , biochemistry , enzyme , hydrolase , bacterial cell structure , biology , cell wall , transferase , chemistry , microbiology and biotechnology , bacteria , genetics
Muramidases/lysozymes hydrolyse the peptidoglycan component of the bacterial cell wall. They are found in many of the glycoside hydrolase (GH) families. Family GH25 contains muramidases/lysozymes, known as CH type lysozymes, as they were initially discovered in the Chalaropsis species of fungus. The characterized enzymes from GH25 exhibit both β-1,4- N -acetyl- and β-1,4- N ,6-O-diacetylmuramidase activities, cleaving the β-1,4-glycosidic bond between N -acetylmuramic acid (NAM) and N -acetylglucosamine (NAG) moieties in the carbohydrate backbone of bacterial peptidoglycan. Here, a set of fungal GH25 muramidases were identified from a sequence search, cloned and expressed and screened for their ability to digest bacterial peptidoglycan, to be used in a commercial application in chicken feed. The screen identified the enzyme from Acremonium alcalophilum JCM 736 as a suitable candidate for this purpose and its relevant biochemical and biophysical and properties are described. We report the crystal structure of the A . alcalophilum enzyme at atomic, 0.78 Å resolution, together with that of its homologue from Trichobolus zukalii at 1.4 Å, and compare these with the structures of homologues. GH25 enzymes offer a new solution in animal feed applications such as for processing bacterial debris in the animal gut.