Open AccessConvergent antibody evolution and clonotype expansion following influenza virus vaccination
Author(s) -
David Forgacs,
Rodrigo B. Abreu,
Giuseppe A. Sautto,
Greg A. Kirchenbaum,
Elliott F. Drábek,
Kevin Williamson,
Dongkyoon Kim,
Daniel Emerling,
Ted M. Ross
Publication year - 2021
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0247253
Subject(s) - hemagglutinin (influenza) , virology , antibody repertoire , biology , vaccination , repertoire , virus , antibody , immune system , breakpoint cluster region , immunology , influenza a virus , gene , genetics , physics , acoustics
Recent advances in high-throughput single cell sequencing have opened up new avenues into the investigation of B cell receptor (BCR) repertoires. In this study, PBMCs were collected from 17 human participants vaccinated with the split-inactivated influenza virus vaccine during the 2016–2017 influenza season. A combination of Immune Repertoire Capture (IRC TM ) technology and IgG sequencing was performed on ~7,800 plasmablast (PB) cells and preferential IgG heavy-light chain pairings were investigated. In some participants, a single expanded clonotype accounted for ~22% of their PB BCR repertoire. Approximately 60% (10/17) of participants experienced convergent evolution, possessing public PBs that were elicited independently in multiple participants. Binding profiles of one private and three public PBs confirmed they were all subtype-specific, cross-reactive hemagglutinin (HA) head-directed antibodies. Collectively, this high-resolution antibody repertoire analysis demonstrated the impact evolution can have on BCRs in response to influenza virus vaccination, which can guide future universal influenza prophylactic approaches.