Open AccessWhole-genome analyses of extended-spectrum or AmpC β-lactamase-producing Escherichia coli isolates from companion dogs in Japan
Author(s) -
Mayo Yasugi,
Shingo Hatoya,
Daisuke Motooka,
Yuki Matsumoto,
Shunsuke Shimamura,
Hiroyuki Tani,
Masaru Furuya,
Keiichiro Mie,
Masami Miyake,
Shota Nakamura,
Terumasa Shimada
Publication year - 2021
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0246482
Subject(s) - biology , escherichia coli , plasmid , enterobacteriaceae , feces , microbiology and biotechnology , genome , clade , homology (biology) , genetics , phylogenetics , virology , gene
The emergence and global spread of extended-spectrum or AmpC β-lactamase (ESBL/AmpC)-producing Enterobacteriaceae in companion animals have led to the hypothesis that companion animals might be reservoirs for cross-species transmission because of their close contact with humans. However, current knowledge in this field is limited; therefore, the role of companion animals in cross-species transmission remains to be elucidated. Herein, we studied ESBL/AmpC-producing Enterobacteriaceae, Escherichia coli in particular, isolated from extraintestinal sites and feces of companion dogs. Whole-genome sequencing analysis revealed that (i) extraintestinal E . coli isolates were most closely related to those isolated from feces from the same dog, (ii) chromosomal sequences in the ST131/C1-M27 clade isolated from companion dogs were highly similar to those in the ST131/C1-M27 clade of human origin, (iii) certain plasmids, such as IncFII/pMLST F1:A2:B20/ bla CTX-M-27 , IncI1/pMLST16/ bla CTX-M-15 , or IncI1/ bla CMY-2 from dog-derived E . coli isolates, shared high homology with those from several human-derived Enterobacteriaceae, (iv) chromosomal bla CTX-M-14 was identified in the ST38 isolate from a companion dog, and (v) eight out of 14 tested ESBL/AmpC-producing E . coli isolates (i.e., ST131, ST68, ST405, and ST998) belonged to the human extraintestinal pathogenic E . coli (ExPEC) group. All of the bla -coding plasmids that were sequenced genome-wide were capable of horizontal transfer. These results suggest that companion dogs can spread ESBL/AmpC-producing ExPEC via their feces. Furthermore, at least some ESBL/AmpC-producing ExPECs and bla -coding plasmids can be transmitted between humans and companion dogs. Thus, companion dogs can act as an important reservoir for ESBL/AmpC-producing E . coli in the community.