Open AccessPhysicochemical properties and formulation development of a novel compound inhibiting Staphylococcus aureus biofilm formation
Author(s) -
Nan Wang,
Qi Feng,
Haqing Yu,
Bryan D. Yestrepsky,
Scott D. Larsen,
Honglan Shi,
Juan Ji,
David W. Anderson,
Hao Li,
Hongmin Sun
Publication year - 2021
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0246408
Subject(s) - biofilm , virulence , staphylococcus aureus , microbiology and biotechnology , solubility , chemistry , virulence factor , antibiotic resistance , drug resistance , antibiotics , bacteria , biology , biochemistry , gene , organic chemistry , genetics
The emergence of antibiotic resistance over the past several decades has given urgency to new antibacterial strategies that apply less selective pressure. A new class of anti-virulence compounds were developed that are active against methicillin-resistant Staphylococcus aureus (MRSA), by inhibiting bacterial virulence without hindering their growth to reduce the selective pressure for resistance development. One of the compounds CCG-211790 has demonstrated potent anti-biofilm activity against MRSA. This new class of anti-virulence compounds inhibited the gene expression of virulence factors involved in biofilm formation and disrupted the biofilm structures. In this study, the physicochemical properties of CCG-211790, including morphology, solubility in pure water or in water containing sodium dodecyl sulfate, solubility in organic solvents, and stability with respect to pH were investigated for the first time. Furthermore, a topical formulation was developed to enhance the therapeutic potential of the compound. The formulation demonstrated acceptable properties for drug release, viscosity, pH, cosmetic elegance and stability of over nine months.