Open AccessGibberellin induced transcription factor bZIP53 regulates CesA1 expression in maize kernels
Author(s) -
Huayang Lv,
Li Xiao,
Hui Li,
YuFeng Hu,
Hanmei Liu,
Shengjuan Wen,
Yangping Li,
Yanping Liu,
Huanhuan Huang,
Guowu Yu,
Yubi Huang,
Junjie Zhang
Publication year - 2021
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0244591
Subject(s) - gibberellin , transcription factor , gene expression , biology , gene , leucine zipper , transcriptome , transcription (linguistics) , cellulose , microbiology and biotechnology , biochemistry , botany , philosophy , linguistics
Proper development of the maize kernel is of great significance for high and stable maize yield to ensure national food security. Gibberellin (GA), one of the hormones regulating plant growth, is involved in modulating the development of maize kernels. Cellulose, one of the main components of plant cells, is also regulated by gibberellin. The mechanism of hormone regulation during maize grain development is highly complicated, and reports on GA-mediated modulation of cellulose synthesis during maize grain development are rare. Our study revealed that during grain growth and development, the grain length and bulk density of GA-treated corn kernels improved significantly, and the cellulose content of grains increased, while seed coat thickness decreased. The transcription factor basic region/leucine zipper motif 53 ( bZIP53) , which is strongly correlated with cellulose synthase gene 1 ( CesA1) expression, was screened by transcriptome sequencing and the expression of the cellulose synthase gene in maize grain development after GA treatment was determined. It was found that bZIP53 expression significantly promoted the expression of CesA1 . Further, analysis of the transcription factor bZIP53 determined that the gene-encoded protein was localized in the cell and nuclear membranes, but the transcription factor bZIP53 itself showed no transcriptional activation. Further studies are required to explore the interaction of bZIP53 with CesA1 .