Open AccessSpectroscopic and microscopic examination of teeth exposed to green tea at different temperatures
Author(s) -
Sinai H. C. Manno,
Francis A. M. Manno,
Li Tian,
Muhammad Shehzad Khan,
Irfan Ahmed,
Yuanchao Liu,
Vincent Wai Tsun Li,
Shisan Xu,
Fangjing Xie,
TaiFeng Hung,
Victor Ma,
William Cho,
Beatriz Aldape,
Shuk Han Cheng,
Condon Lau
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0244542
Subject(s) - enamel paint , demineralization , in vivo , chemistry , scanning electron microscope , inductively coupled plasma , green tea , materials science , food science , composite material , biology , plasma , microbiology and biotechnology , physics , quantum mechanics
Tea is a popular beverage consumed at different temperatures. The effect of tea on teeth at different temperatures has not been studied previously. The present study used an in vitro green tea immersed tooth model at different tea temperatures (hot and cold) compared to an in vivo tea administration model allowing rats to drink tea over the course of a week. The elements present in tea leaves were identified by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) and compared to the elements in teeth (enamel surface) using Laser-Induced Breakdown Spectroscopy (LIBS). Here, LIBS demonstrated in vivo and in vitro green tea treatments resulted in a significant increase in the mineral elements found in enamel. For the in vitro assessment, elements in enamel varied based on cold-tea and hot-tea treatment; however, hot water reduced the elements in enamel. Atomic force microscopy found the in vivo tea group had a higher roughness average (RA) compared with the in vivo water group. Cold tea and hot tea in vitro groups demonstrated lower RA than in vitro water controls. Scanning electron microscopy found hot water induced cracks more than 1.3μm in enamel while cold tea and hot tea promoted the adhering of extrinsic matter to teeth. Overall, teeth treated to high temperature lost the mineral phase leading to demineralization. Our results indicate that green tea protects enamel, but its protective action in dental structures is enhanced at cold temperature.