Open AccessIdentification and characterization of a novel multi-stress responsive gene in Arabidopsis
Author(s) -
Faiza Tawab,
Iqbal Munir,
Zeeshan Nasim,
Mohammad Sayyar Khan,
Saleha Tawab,
Adnan Nasim,
Aqib Iqbal,
Mian Afaq Ahmad,
Waqar Ali,
Raheel Munir,
Maria Munir,
Noreen Asim
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0244030
Subject(s) - abscisic acid , abiotic stress , salinity , arabidopsis , germination , mutant , biology , transcriptome , genetically modified crops , gene , abiotic component , transgene , glycine , gene expression , botany , microbiology and biotechnology , horticulture , genetics , ecology , paleontology , amino acid
Abiotic stresses especially salinity, drought and high temperature result in considerable reduction of crop productivity. In this study, we identified AT4G18280 annotated as a glycine-rich cell wall protein-like (hereafter refer to as GRPL1 ) protein as a potential multistress-responsive gene. Analysis of public transcriptome data and GUS assay of pGRPL1 :: GUS showed a strong induction of GRPL1 under drought, salinity and heat stresses. Transgenic plants overexpressing GRPL1 -3HA showed significantly higher germination, root elongation and survival rate under salt stress. Moreover, the 35S :: GRPL1-3HA transgenic lines also showed higher survival rates under drought and heat stresses. GRPL1 showed similar expression patterns with Abscisic acid (ABA)-pathway genes under different growth and stress conditions, suggesting a possibility that GRPL1 might act in the ABA pathway that is further supported by the inability of ABA-deficient mutant ( aba2-1 ) to induce GRPL1 under drought stress. Taken together, our data presents GRPL1 as a potential multi-stress responsive gene working downstream of ABA.