Open AccessStable and efficient immobilization of bi-enzymatic NADPH cofactor recycling system under consecutive microwave irradiation
Author(s) -
Rong Chen,
Qiuhui Wei,
Xin Wei,
Yuheng Liu,
Xiaomin Zhang,
Xiabin Chen,
Xia Yin,
Tian Xie
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0242564
Subject(s) - cofactor , chemistry , enzyme , microwave irradiation , biochemistry , irradiation , biophysics , biology , catalysis , physics , nuclear physics
One of the challenges in biocatalysis is the development of stable and efficient bi-enzymatic cascades for bio-redox reactions coupled to the recycling of soluble cofactors. Aldo-keto reductase (LEK) and glucose dehydrogenase (GDH) can be utilized as the NADPH recycling system for economic and efficient biocatalysis of ( R )-4-chloro-3-hydroxybutanoate (( R )-CHBE), an important chiral pharmaceutical intermediate. The LEK and GDH was efficiently co-immobilized in mesocellular siliceous foams (MCFs) under microwave irradiation (CoLG-MIA). while they were also co-immobilized by entrapment in calcium alginate without MIA as control (CoLG-CA). The relative activity of CoLG-MIA was increased to 140% compared with that of free LEK. The CoLG-MIA exhibited a wider range of pH and temperature stabilities compared with other preparations. The thermal, storage and batch operational stabilities of microwave-assisted immobilized LEK-GDH were also improved. The NADPH recycling system exhibited the potential as the stable and efficient catalyst for the industrial preparation of ( R )-CHBE.