Open AccessIn vivo tracking of transplanted macrophages with near infrared fluorescent dye reveals temporal distribution and specific homing in the liver that can be perturbed by clodronate liposomes
Author(s) -
Satoshi Nishiwaki,
Shigeki Saito,
Kyosuke Takeshita,
Hidefumi Kato,
Ryuzo Ueda,
Akiyoshi Takami,
Tomoki Naoe,
Mika Ogawa,
Takayuki Nakayama
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0242488
Subject(s) - spleen , homing (biology) , phagocytosis , in vivo , liposome , macrophage , lung , bone marrow , microbiology and biotechnology , innate immune system , chemistry , pathology , biology , immunology , medicine , in vitro , immune system , biochemistry , ecology
Macrophages play an indispensable role in both innate and acquired immunity, while the persistence of activated macrophages can sometimes be harmful to the host, resulting in multi-organ damage. Macrophages develop from monocytes in the circulation. However, little is known about the organ affinity of macrophages in the normal state. Using in vivo imaging with XenoLight DiR®, we observed that macrophages showed strong affinity for the liver, spleen and lung, and weak affinity for the gut and bone marrow, but little or no affinity for the kidney and skin. We also found that administered macrophages were still alive 168 hours after injection. On the other hand, treatment with clodronate liposomes, which are readily taken up by macrophages via phagocytosis, strongly reduced the number of macrophages in the liver, spleen and lung.