Open AccessS-adenosyl methionine synthetase SAMS-5 mediates dietary restriction-induced longevity in Caenorhabditis elegans
Author(s) -
Chia Chang Chen,
Chiao Yin Lim,
Pin Jung Lee,
Ao Lin Hsu,
Tsui Ting Ching
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0241455
Subject(s) - caenorhabditis elegans , longevity , methionine , biology , epistasis , phenotype , methionine adenosyltransferase , microbiology and biotechnology , genetics , biochemistry , gene , amino acid
S-adenosyl methionine synthetase (SAMS) catalyzes the biosynthesis of S-adenosyl methionine (SAM), which serves as a universal methyl group donor for numerous biochemical reactions. Previous studies have clearly demonstrated that SAMS-1, a C . elegans homolog of mammalian SAMS, is critical for dietary restriction (DR)-induced longevity in Caenorhabditis elegans . In addition to SAMS-1, three other SAMS paralogs have been identified in C . elegans . However, their roles in longevity regulation have never been explored. Here, we show that depletion of sams-5 , but not sams-3 or sams-4 , can extend lifespan in worms. However, the phenotypes and expression pattern of sams-5 are distinct from sams-1 , suggesting that these two SAMSs might regulate DR-induced longevity via different mechanisms. Through the genetic epistasis analysis, we have identified that sams-5 is required for DR-induced longevity in a pha-4/FOXA dependent manner.