Open AccessMolecular characterization of clinical and environmental Vibrio parahaemolyticus isolates in Huzhou, China
Author(s) -
Wei Yan,
Lei Ji,
Deshun Xu,
Liping Chen,
Xiaofang Wu
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0240143
Subject(s) - virulence , microbiology and biotechnology , vibrio parahaemolyticus , serotype , pulsed field gel electrophoresis , tetracycline , biology , typing , kanamycin , antibiotic resistance , genotype , ampicillin , antibiotics , bacteria , gene , genetics
Vibrio parahaemolyticus is responsible for seafood-borne gastroenteritis worldwide. Isolates of V . parahaemolyticus from clinical samples (n = 54) and environmental samples (n = 38) in Huzhou were analyzed by serological typing, virulence gene detection, antibiotic resistance testing, and pulsed-field gel electrophoresis (PFGE) for molecular typing. O3:K6 was the main serotype and tlh+tdh+trh- was the most frequently detected virulence genotype in clinical strains. O2:Kut was the main serotype and tlh+tdh-trh- was the most frequently detected virulence genotype in environmental strains. Antibiotic resistance testing indicated that the isolates were highly resistant to ampicillin (90.76%), followed by gentamicin and tetracycline. Following the restriction enzyme Not I digestion, the 91 strains yielded 81 PFGE patterns, and 16 clones had similarity values of > 85.00%, indicating a high level of diversity. Finally, there may be cross-contamination between freshwater and seawater products, so it is necessary to strengthen supervision of food processing.