Open AccessMotion magnification analysis of microscopy videos of biological cells
Author(s) -
Oren Shabi,
Sari Natan,
Avraham Kolel,
Angshuman Mukherjee,
Oren Tchaicheeyan,
Haguy Wolfenson,
Nahum Kiryati,
Ayelet Lesman
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0240127
Subject(s) - beat (acoustics) , magnification , oscillation (cell signaling) , microscopy , video microscopy , lens (geology) , motion analysis , biological motion , rhythm , computer vision , physics , computer science , biological system , biology , optics , motion (physics) , acoustics , microbiology and biotechnology , genetics
It is well recognized that isolated cardiac muscle cells beat in a periodic manner. Recently, evidence indicates that other, non-muscle cells, also perform periodic motions that are either imperceptible under conventional lab microscope lens or practically not easily amenable for analysis of oscillation amplitude, frequency, phase of movement and its direction. Here, we create a real-time video analysis tool to visually magnify and explore sub-micron rhythmic movements performed by biological cells and the induced movements in their surroundings. Using this tool, we suggest that fibroblast cells perform small fluctuating movements with a dominant frequency that is dependent on their surrounding substrate and its stiffness.