Open AccessGenomic approach for conservation and the sustainable management of endangered species of the Amazon
Author(s) -
Paola Fabiana Fazzi-Gomes,
Jonas Aguiar,
Gleyce Fonseca Cabral,
Diego Marques,
Helber Gonzales Almeida Palheta,
Fabiano Cordeiro Moreira,
Marília Danyelle Nunes Rodrigues,
Roberta Barroso Cavalcante,
Jorge Estefano Santana de Souza,
Caio Santos Silva,
Igor Guerreiro Hamoy,
Sidney Emanuel Batista dos Santos
Publication year - 2021
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0240002
Subject(s) - microsatellite , biology , endangered species , conservation genetics , amazon rainforest , aquaculture , evolutionary biology , genomics , fisheries management , fishing , genome , ecology , zoology , fishery , genetics , fish <actinopterygii> , gene , allele , habitat
A broad panel of potentially amplifiable microsatellite loci and a multiplex system were developed for the Amazonian symbol fish species Arapaima gigas, which is currently in high danger of extinction due to the disorderly fishing exploitation. Several factors have contributed to the increase of this threat, among which we highlight the lack of genetic information about the structure and taxonomic status of the species, as well as the lack of accurate tools for evaluation of the effectivity of current management programs. Based on Arapaima gigas’ whole genome, available at the NCBI database (ID: 12404), a total of 95,098 unique perfect microsatellites were identified, including their proposed primers. From this panel, a multiplex system containing 12 tetranucleotide microsatellite markers was validated. These tools are valuable for research in as many areas as bioinformatics, ecology, genetics, evolution and comparative studies, since they are able to provide more accurate information for fishing management, conservation of wild populations and genetic management of aquaculture.