Open AccessMolecular evidence of the hybrid origin of Cryptocoryne ×purpurea Ridl. nothovar. purpurea (Araceae)
Author(s) -
Rusly Rosazlina,
N. Kingo Jacobsen,
Marian Ørgaard,
Ahmad Sofiman Othman
Publication year - 2021
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0239499
Subject(s) - biology , hybrid , internal transcribed spacer , botany , chloroplast dna , sympatric speciation , ribosomal dna , pollen , araceae , ribosomal rna , chloroplast , genetics , phylogenetics , gene
Natural hybridization has been considered a source of taxonomic complexity in Cryptocoryne . A combined study of DNA sequencing data from the internal transcribed spacer (ITS) of nuclear ribosomal DNA and the trn K -mat K region of chloroplast DNA was used to identify the parents of Cryptocoryne putative hybrids from Peninsular Malaysia. Based on the intermediate morphology and sympatric distribution area, the plants were tentatively identified as the hybrid Cryptocoryne × purpurea nothovar. purpurea . The plants were pollen sterile and had long been considered as hybrids, supposedly between two related and co-existing species, C . cordata var. cordata and C . griffithii . The status of C . × purpurea nothovar. purpurea was independently confirmed by the presence of an additive ITS sequence pattern from these two parental species in hybrid individuals. An analysis of the chloroplast trn K -mat K sequences showed that the hybridization is bidirectional with the putative hybrids sharing identical sequences from C . cordata var. cordata and C . griffithii , indicating that both putative parental species had been the maternal parent in different accessions.