Open AccessPolydopamine-assisted PDGF-BB immobilization on PLGA fibrous substrate enhances wound healing via regulating anti-inflammatory and cytokine secretion
Author(s) -
Xiao Yang,
Peng Zhan,
Xiuyan Wang,
Qiushuang Zhang,
Yi Zhang,
Haitao Fan,
Ranwei Li,
Ming Zhang
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0239366
Subject(s) - wound healing , platelet derived growth factor receptor , cytokine , angiogenesis , plga , platelet derived growth factor , growth factor , chemokine , pharmacology , medicine , chemistry , microbiology and biotechnology , inflammation , immunology , cancer research , in vitro , biochemistry , biology , receptor
Platelet-derived growth factor-bb (PDGF-BB) is a potent chemokine and mitogen for fibroblasts, keratinocytes, and vascular endothelium in the injured area, believed to be effective in wound healing. However, the short half-life of PDGF-BB and its rapid release from the wound surface limited its efficacy in vivo and vitro . To evaluate the wound healing effects of dorsal skin in SD rats with polydopamine-assisted immobilized PDGF-BB on PLGA nanofibrous substrate. First, the effects of pDA-coating and PDGF-BB immobilization on the morphology, compositions, and hydrophilicity of substrates were evaluated in details. Second, the wound healing effect of pDA/PLGA/PDGF-BB substrate was assessed in the dorsal skin of SD rats. Last, the cytokine analysis by ELISA method was employed to evaluate the advantages of pDA/PLGA/PDGF-BB substrate on anti-inflammatory, angiogenesis, and cellular proliferation. This method significantly improved the immobilization amount and stability of PDGF-BB on the substrate ( p <0.01), further improved the hydrophilicity of substrates ( p <0.05). Furthermore, the wound closure process was much more accelerated in the pDA/PLGA/PDGF-BB group ( p <0.05). H&E and CD31 staining informed that the wound treated by pDA/PLGA/PDGF-BB substrate showed a high degree of regeneration and angiogenesis. The cytokine analysis showed that pDA significantly reduced the high level of inflammatory cytokines such as TNF-α ( p <0.05). And the immobilized PDGF-BB significantly elevated the level of TGF-β and VEGF ( p <0.05). The pDA/PLGA/PDGF-BB substrate showed great therapeutic effect on wound healing compared with other control groups via regulating anti-inflammatory, angiogenesis, and cellular proliferation. Absolutely, this report offered an available novel method for skin regeneration.