Open AccessCD32 is enriched on CD4dimCD8bright T cells
Author(s) -
Amber K Virdi,
Jennillee Wallace,
Hannah J. Barbian,
Maureen Richards,
Ethan Ritz,
Beverly E. Sha,
Lena AlHarthi
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0239157
Subject(s) - flow cytometry , cd8 , cytotoxic t cell , population , context (archaeology) , interleukin 21 , t cell , biology , immunology , microbiology and biotechnology , medicine , in vitro , immune system , biochemistry , paleontology , environmental health
CD4 dim CD8 bright T cells, a genuine population of CD8 + T cells, are highly activated and cytolytic. Recently, the low affinity IgG Fc fragment receptor CD32a was described as marker of HIV latency while others reported that CD32a is associated with T cell activation. Given that we have previously established that CD4 dim CD8 bright T cells are highly activated, mediate anti-HIV responses, and are infected by HIV, we assessed here CD32 expression on CD4 dim CD8 bright T cells in context of HIV. CD32 frequency on peripheral CD4 dim CD8 bright and CD4 + T cells was determined by flow cytometry among HIV negative and HIV positive patients. We report that among HIV - individuals, mean CD32 percent expression was 60% on CD4 dim CD8 bright T cells and 17% on CD4 + T cells (p<0.01). Among HIV + patients, mean CD32 percent expression was 54% on CD4 dim CD8 bright T cells and 12% on CD4 + T cells (p<0.001). CD32 expression on CD4 dim CD8 bright T cells did not correlate with CD4 count and viral load and was not different by HIV serostatus. CD32 was also higher on other double positive T cell populations in both HIV negative and HIV positive donors in comparison to their single positive T cell counterpart. Together, these studies indicate that CD32 is enriched on double positive T cells regardless of HIV serostatus. The functional role of CD32 on these double positive T cells remains to be elucidated.