Open AccessEvaluation of the GenoType NTM-DR assay performance for the identification and molecular detection of antibiotic resistance in Mycobacterium abscessus complex
Author(s) -
Nicolas Bouzinbi,
Olivier Marcy,
Thibault Bertolotti,
R. Chiron,
Pascale Bémer,
Martine Pestel-Caron,
Olivia Peuchant,
Hélène Guet-Revillet,
Marie-Sarah Fangous,
Geneviève Héry-Arnaud,
Abdoul–Salam Ouédraogo,
AnneLaure Bañuls,
Sylvain Godreuil
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0239146
Subject(s) - mycobacterium abscessus , amikacin , genotype , biology , clarithromycin , microbiology and biotechnology , nontuberculous mycobacteria , broth microdilution , subspecies , multilocus sequence typing , drug resistance , genotyping , genetics , antibiotics , mycobacterium , gene , minimum inhibitory concentration , bacteria , paleontology
The first objective of this study was to determine the GenoType NTM-DR assay performance for subspecies identification in Mycobacterium abscessus complex isolates. The second objective was to evaluate the GenoType NTM-DR assay ability to detect clarithromycin and amikacin resistance in M . abscessus complex isolates compared with drug susceptibility testing (DST) and PCR sequencing of the erm(41) , rrl and rrs genes. The concordance between the GenoType NTM-DR and MLST results concerning subspecies identification was 100%. The wild type and mutated alleles of the rrl and rrs genes were detected by the GenoType NTM-DR assay and PCR sequencing with 100% (115/115) agreement. Similarly, 100% concordance between GenoType NTM-DR and DST was observed for clarithromycin and amikacin testing. Sensitivity for the detection of clarithromycin and amikacin resistance was 100%. The GenoType NTM-DR assay provides a robust and complementary tool to the gold standard methods (MLST and broth microdilution) for subspecies identification and drug resistance detection.