Open AccessiBRAB: In silico based-designed broad-spectrum Fab against H1N1 influenza A virus
Author(s) -
Phuc-Chau Do,
Trung Hai Nguyen,
Uyen Vo,
Ly Le
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0239112
Subject(s) - in silico , virus , virology , influenza a virus , broad spectrum , outbreak , sars virus , covid-19 , biology , computational biology , medicine , genetics , chemistry , infectious disease (medical specialty) , disease , pathology , gene , combinatorial chemistry
Influenza virus A is a significant agent involved in the outbreak of worldwide epidemics, causing millions of fatalities around the world by respiratory diseases and seasonal illness. Many projects had been conducting to investigate recovered infected patients for therapeutic vaccines that have broad-spectrum activity. With the aid of the computational approach in biology, the designation for a vaccine model is more accessible. We developed an in silico protocol called i BRAB to design a broad-reactive Fab on a wide range of influenza A virus. The Fab model was constructed based on sequences and structures of available broad-spectrum Abs or Fabs against a wide range of H1N1 influenza A virus. As a result, the proposed Fab model followed i BRAB has good binding affinity over 27 selected HA of different strains of H1 influenza A virus, including wild-type and mutated ones. The examination also took by computational tools to fasten the procedure. This protocol could be applied for a fast-designed therapeutic vaccine against different types of threats.