Open AccessAssociation of IL16 polymorphisms with periodontitis in Brazilians: A case- control study
Author(s) -
Victor Hugo de Souza,
Jeane Eliete Laguila Visentainer,
Joana Maira Valentini Zacarias,
Josiane Bazzo de Alencar,
Patrícia Yumeko Tsuneto,
Cléverson O. Silva,
Samira Salmeron,
Cristiane Maria Colli,
Ana Maria Sell
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0239101
Subject(s) - genotyping , genotype , haplotype , periodontitis , chronic periodontitis , medicine , polymorphism (computer science) , allele , interleukin 17 , immunology , immune system , genetics , biology , gene
Periodontitis (PD) is a chronic inflammatory process resulting from the relationship of the immune response with the components in dental plaque. Cytokines and their genetic polymorphisms seem to be involved in the immunopathogenesis of this disease. This study aimed to evaluate the correlation of IL16 polymorphism with PD. A case-control study was conducted in a sample of individuals from southern Brazil. The genotyping of IL16 , rs11556218 T>G , rs4072111 C>T e rs4778889 T>C , was performed using the PCR-RFLP methodology. The serum level of IL-16 was determined using an IL-16 ELISA kit for humans. SNPStats and OpenEpi software and Wilcoxon's U test were used to perform statistical analysis. IL16 rs11556218 polymorphism was significantly associated to PD in nonsmoking patients: individuals with G/G genotype were less likely to develop PD compared to the T/T genotype (OR = 0.10; Pc = 0.019, codominant model). In addition, the TTT haplotype was associated with a high risk for PD (OR = 2.45; P = 0.01). A low IL-16 serum level was observed among individuals with PD when compared to controls ( P = 0.027). Thus, the IL16 rs16556218 polymorphism and the serum levels of IL-16 were associated with periodontitis in a Brazilian sample, and this was influenced by environmental factors such as smoking.