Open AccessEfficacy of six disinfection methods against extended-spectrum beta-lactamase (ESBL) producing E. coli on eggshells in vitro
Author(s) -
Gerzon Motola,
Hafez Mohamed Hafez,
Sarah Brüggemann-Schwarze
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0238860
Subject(s) - contamination , eggshell , peracetic acid , disinfectant , microbiology and biotechnology , formaldehyde , escherichia coli , human decontamination , food science , hydrogen peroxide , antimicrobial , biology , chemistry , medicine , ecology , organic chemistry , pathology , gene , biochemistry
The presence of extended-spectrum beta-lactamase (ESBL) producing Escherichia coli on poultry products is an important issue for veterinary and human health due to the zoonotic infection risk for producers and consumers. The present study focuses on testing the efficacy of six different disinfection methods on eggshell samples, aiming to reduce ESBL producing E . coli contamination on the hatching egg. Sterile eggshell cutouts were artificially contaminated with 10 8 cfu/ml CTX-M-1 producing E . coli and used as a carrier model to analyze the efficacy of six disinfection methods. The contaminated samples were separated into two groups; 1) contaminated and disinfected, 2) contaminated and non-disinfected. Six independent disinfection protocols were performed following product specifications and protocols. Each eggshell sample was separately crushed, and the total viable bacterial count was calculated to determine the disinfection efficacy. Five out of six tested methods (formaldehyde gassing, hydrogen peroxide + alcohol spray, essential oils spray, peracetic acid foam, and low energetic electron radiation) demonstrated a reduction or completely eliminated the initial ESBL producing E . coli contamination. One method (essential oils as cold fog) only partly reached the expected efficacy threshold (reduction of >10 2 cfu/ml) and the result differed significantly when compared to the reference method i.e. formaldehyde gassing.