Open AccessChanges of vaginal microbiota during cervical carcinogenesis in women with human papillomavirus infection
Author(s) -
Kyeong A So,
Eun Jung Yang,
Nae Ry Kim,
Sung Ran Hong,
Jae-Ho Lee,
Chan-Soo Hwang,
SeungHyuk Shim,
Sun Joo Lee,
Tae Jin Kim
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0238705
Subject(s) - cervical cancer , prevotella , lactobacillus crispatus , gardnerella vaginalis , cervical intraepithelial neoplasia , medicine , bacterial vaginosis , lactobacillus , anaerobic bacteria , gynecology , lactobacillus gasseri , hpv infection , oncology , cancer , gastroenterology , biology , bacteria , genetics
Objective To evaluate the changes of vaginal microbiota during cervical carcinogenesis in women with high-risk human papillomavirus infection. Materials and methods Vaginal microbiota was analyzed using next-generation sequencing in women with normal, cervical intraepithelial neoplasia (CIN), or cervical cancer. Results A marked decrease of Lactobacillus crispatus was found in the CIN/cancer groups compared with that in the normal group. The diversity of microorganisms increased in patients with CIN or cervical cancer with HPV infection. Atopobium vaginae (OR 4.33, 95% CI 1.15–16.32), Dialister invisus (OR 4.89, 95% CI 1.20–19.94), Finegoldia magna (OR 6.00, 95% CI 1.08–33.27), Gardnerella vaginalis (OR 7.43, 95% CI 1.78–31.04), Prevotella buccalis (OR 11.00, 95% CI 2.00–60.57), and Prevotella timonensis (OR 6.00, 95% CI 1.46–24.69) were significantly associated with the risk of CIN 2/3 or cervical cancer. Conclusion Women with the CIN and cervical cancer showed a high diversity in vaginal microbiota. Depletion of Lactobacillus crispatus and increased abundance of anaerobic bacteria were detected in women with cervical disease.