Open AccessRetention of lumpy skin disease virus in Stomoxys spp (Stomoxys calcitrans, Stomoxys sitiens, Stomoxys indica) following intrathoracic inoculation, Diptera: Muscidae
Author(s) -
Arman Issimov,
David B. Taylor,
Malik Shalmenov,
Birzhan Nurgaliyev,
Izimgali Zhubantayev,
Nurzhan Abekeshev,
Kaissar Kushaliyev,
Kereyev Abzal,
Lespek Kutumbetov,
Assylbek Zhanabayev,
Yasmin Zhakiyanova,
Peter White
Publication year - 2021
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0238210
Subject(s) - stomoxys , muscidae , biology , virology , virus , inoculation , pathogen , microbiology and biotechnology , immunology , botany
Lumpy skin disease (LSD) is an emerging disease of cattle in Kazakhstan and the means of transmission remains uncertain. In the current study, retention of Lumpy Skin Disease Virus (LSDV) by three Stomoxys species following intrathoracic inoculation was demonstrated under laboratory conditions. A virulent LSDV strain was injected into the thorax of flies to bypass the midgut barrier. The fate of the pathogen in the hemolymph of the flies was examined using PCR and virus isolation tests. LSDV was isolated from all three Stomoxys species up to 24h post inoculation while virus DNA was detectable up to 7d post inoculation.