Open AccessGrape juice attenuates left ventricular hypertrophy in dyslipidemic mice
Author(s) -
Ângela Maria Martins,
Danielle Aparecida Quintino Silva Sarto,
Karine de Paula Caproni,
Janaína Silva,
Jaqueline Silva,
Paulo Sérgio Lopes de Souza,
Leandro dos Santos,
Marcos Javier Espino Ureña,
Maria das Graças de Souza Carvalho,
Brígida Monteiro Vilas Boas,
Lidiane Paula Ardisson Miranda,
José Antônio Dias Garcia
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0238163
Subject(s) - simvastatin , dyslipidemia , medicine , endocrinology , insulin resistance , muscle hypertrophy , chemistry , left ventricular hypertrophy , lipid profile , cholesterol , diabetes mellitus , blood pressure
Objective We evaluated the effects of grape juice ( Vitis labrusca L.) on dyslipidemia, resistance to insulin, and left ventricular hypertrophy (LVH) in mice homozygous for the absence of the LDL receptor gene (LDLr -/-) under a hyperlipidemic diet. Methodology We divided 30 male mice (3 months old) into three groups (n = 10); the HL group was fed a high-fat diet, the HLU group received a high-fat diet and 2 g/kg/day of grape juice, and the HLS group was fed a high-fat diet and simvastatin (20 mg/kg/day). We assessed the blood pressure profile of the mice. We also determined the levels of C-reactive protein (CRP) and lipid profile, glycemic and insulinemic profiles, and calculated the HOMA-IR. Cardiomyocyte hypertrophy, interstitial collagen deposit, and the expression of CD40 ligand (CD40L) and metalloproteinases 2 and 9 were assessed immunohistologically. Results After 60 days, the mice treated with grape juice showed similar results as those of the group treated with simvastatin. The use of grape fruit attenuated dyslipidemia and insulin resistance and significantly increased the levels of high cholesterol density lipoproteins (HDLc). The antioxidant potential of phenolic compounds associated with the increase in HDLc levels in the mice of the HLU group prevented the development of LVH and arterial hypertension since it inhibited the inflammatory response induced by the CD40 pathway and its ligand CD40L. Consequently, there was a lower expression of MMP-2 and MMP-9 and lower serum levels of CRP. Conclusion Grape juice has a hypolipidemic and cardiac protective potential, presenting a similar effect as that of simvastatin through a direct antioxidant action of phenolic compounds, or indirectly, via antioxidant action and anti-inflammatory activity of the HDLc. These results suggest that grape juice is a functional food possessing a high potential to prevent cardiovascular diseases.