Open AccessIdentification of immunodominant linear epitopes from SARS-CoV-2 patient plasma
Author(s) -
Lluc Farrera-Soler,
JeanPierre Daguer,
Sofía Barluenga,
Oscar Vadas,
Patrick Cohen,
Sabrina Pagano,
Sabine Yerly,
Laurent Kaiser,
Nicolas Vuilleumier,
Nicolas Winssinger
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0238089
Subject(s) - epitope , furin , proteolysis , virology , coronavirus , antibody , covid-19 , biology , cleavage (geology) , computational biology , immunology , medicine , biochemistry , pathology , enzyme , infectious disease (medical specialty) , disease , paleontology , fracture (geology)
A novel severe acute respiratory syndrome coronavirus (SARS-CoV-2) is the source of a current pandemic (COVID-19) with devastating consequences in public health and economic stability. Using a peptide array to map the antibody response of plasma from healing patients (12) and heathy patients (6), we identified three immunodominant linear epitopes, two of which correspond to key proteolytic sites on the spike protein (S1/S2 and S2’) known to be critical for cellular entry. We show biochemical evidence that plasma positive for the epitope adjacent to the S1/S2 cleavage site inhibits furin-mediated proteolysis of spike.