Open AccessNovel plant flavonoid electrochemical sensor based on in-situ and controllable double-layered membranes modified electrode
Author(s) -
Jing Hu,
Renjie Zhou,
Lin Huang,
Qiuyuan Wei,
Feilong Hu,
Xin Yang
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0237583
Subject(s) - membrane , electrochemical gas sensor , materials science , fourier transform infrared spectroscopy , graphene , electrochemistry , detection limit , electrode , cyclic voltammetry , chemical engineering , nuclear chemistry , analytical chemistry (journal) , nanotechnology , chemistry , chromatography , biochemistry , engineering
Identification and quantification of plant flavonoids are critical to pharmacokinetic study and pharmaceutical quality control due to their distinct pharmacological functions. Here we report on a novel plant flavonoid electrochemical sensor for sensitive and selective detection of dihydromyricetin (DMY) based on double- layered membranes consisting of gold nanoparticles (Au) anchored on reduced graphene oxide (rGO) and molecularly imprinted polymers (MIPs) modified glassy carbon electrode (GCE). Both rGO-Au and MIPs membranes were directly formed on GCE via in-situ electrochemical reduction and polymerization processes step by step. The compositions, morphologies, and electrochemical properties of membranes were investigated with X-ray powder diffractometry (XRD), Fourier transform infrared spectrum (FTIR), Field emission scanning electron microscopy (FESEM) combined with various electrochemical methods. The fabricated electrochemical sensor labeled as GCE│rGO-Au/MIPs exhibited excellent performance in determining of DMY under optimal experimental conditions. A wide linear detection range (LDR) ranges from 2.0×10 −8 to 1.0×10 −4 M together with a low limit of detection (LOD) of 1.2×10 −8 M ( S/N = 3) were achieved. Moreover, the electrochemical sensor was employed to determine DMY in real samples with satisfactory results.