Open AccessA novel chloroplast super-complex consisting of the ATP synthase and photosystem I reaction center
Author(s) -
Satarupa Bhaduri,
Sandeep Singh,
Whitaker Cohn,
S. Saif Hasan,
Julian P. Whitelegge,
William A. Cramer
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0237569
Subject(s) - thylakoid , atp synthase , photosystem i , chloroplast , f atpase , ferredoxin—nadp(+) reductase , cytochrome b6f complex , chemiosmosis , photosynthetic reaction centre , ferredoxin , photosystem ii , photosystem , chemistry , biophysics , electron transport chain , biology , biochemistry , photosynthesis , enzyme , gene
Several ‘super-complexes’ of individual hetero-oligomeric membrane protein complexes, whose function is to facilitate intra-membrane electron and proton transfer and harvesting of light energy, have been previously characterized in the mitochondrial cristae and chloroplast thylakoid membranes. We report the presence of an intra-membrane super-complex dominated by the ATP-synthase, photosystem I (PSI) reaction-center complex and the ferredoxin-NADP + Reductase (FNR) in the thylakoid membrane. The presence of the super-complex has been documented by mass spectrometry, clear-native PAGE and Western Blot analyses. This is the first documented presence of ATP synthase in a super-complex with the PSI reaction-center located in the non-appressed stromal domain of the thylakoid membrane.