Open AccessAnalysis of melatonin regulation of germination and antioxidant metabolism in different wheat cultivars under polyethylene glycol stress
Author(s) -
Dongxiao Li,
William D. Batchelor,
Di Zhang,
Hanxiao Miao,
Hongye Li,
Shijia Song,
Ruiqi Li
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0237536
Subject(s) - melatonin , radicle , germination , peg ratio , superoxide dismutase , antioxidant , seedling , proline , biology , horticulture , chemistry , agronomy , botany , biochemistry , amino acid , endocrinology , finance , economics
Melatonin is effective in enhancing various abiotic stress resistances of plants. However, its underlying mechanisms in drought-resistance in winter wheat ( Triticum aestivum L.) is not clear. The goal of this work was to investigate the effect of melatonin on seed germination and to evaluate leaf antioxidant physiology for two wheat varieties. Experiments included 20% PEG, melatonin plus 20% PEG and a control using two contrasting wheat varieties (JM22– drought sensitive and HG35– drought resistant). Melatonin levels were 0, 1, 10, 100 and 300 μmol L -1 . Results revealed that 300 μmol L -1 of melatonin alleviated the negative effect of water stress on germination and increased radicle length, radicle number, and plumule length of the germinated seeds. Principal component analysis showed a significant change in amino acid content during germination and this change was dependent on melatonin concentration and the variety. Lysine (Lys) content in wheat seeds under the PEG plus 300 μmol L -1 melatonin treatment increased compared with that of the seeds under PEG alone. There was a significant and positive correlation between Lys content and morphological index of germination. During seedling growth, soluble protein was involved in osmotic adjustment and superoxide dismutase (SOD) activity was increased to mitigate the damage in the cytomembrane of JM 22 leaf under 300 μmol L -1 melatonin plus PEG treatment. The effect of melatonin was dependent on SOD activity increasing significantly for HG35—a drought resistant variety. The results of this work lays a foundation for further studies to determine if melatonin can be economically used to mitigate the impact of dry planting conditions on wheat productivity in North China Plain.