Open AccessEffect of FHA and Prn on Bordetella pertussis colonization of mice is dependent on vaccine type and anatomical site
Author(s) -
Anne Zeddeman,
Evi van Schuppen,
Kristianne E. Kok,
Marjolein van Gent,
Kees Heuvelman,
Marieke J Bart,
Han G. J. van der Heide,
Joshua Gillard,
Elles Simonetti,
Marc J. Eleveld,
Fred J. H. van Opzeeland,
Saskia van Selm,
Ronald de Groot,
Marien I. de Jonge,
Frits R. Mooi,
Dimitri A. Diavatopoulos
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0237394
Subject(s) - filamentous haemagglutinin adhesin , pertactin , bordetella pertussis , pertussis vaccine , biology , microbiology and biotechnology , pertussis toxin , virology , whooping cough , frameshift mutation , vaccination , hemagglutinin (influenza) , antibody , immunology , phenotype , gene , immunization , bacteria , genetics , g protein , receptor
Bordetella pertussis vaccine escape mutants that lack expression of the pertussis antigen pertactin (Prn) have emerged in vaccinated populations in the last 10–20 years. Additionally, clinical isolates lacking another acellular pertussis (aP) vaccine component, filamentous hemagglutinin (FHA), have been found sporadically. Here, we show that both whole-cell pertussis (wP) and aP vaccines induced protection in the lungs of mice, but that the wP vaccine was more effective in nasal clearance. Importantly, bacterial populations isolated from the lungs shifted to an FHA-negative phenotype due to frameshift mutations in the fhaB gene. Loss of FHA expression was strongly selected for in Prn-deficient strains in the lungs following aP but not wP vaccination. The combined loss of Prn and FHA led to complete abrogation of bacterial surface binding by aP-induced serum antibodies. This study demonstrates vaccine- and anatomical site-dependent adaptation of B . pertussis and has major implications for the design of improved pertussis vaccines.