Open AccessUrine metabolomics of rats with chronic atrophic gastritis
Author(s) -
Guoxiu Zu,
Qiang Sun,
Jian Chen,
Xijian Liu,
Keyun Sun,
Liangkun Zhang,
Ling Li,
Tao Han,
Hailiang Huang
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0236203
Subject(s) - urine , purine metabolism , metabolic pathway , metabolomics , atrophic gastritis , glutamine , metabolism , pathogenesis , chemistry , medicine , pharmacology , biochemistry , pathology , gastritis , stomach , chromatography , enzyme , amino acid
Background/aim To use liquid chromatography-mass spectrometry (LC-MS) to identify endogenous differential metabolites in the urine of rats with chronic atrophic gastritis (CAG). Materials and methods Methylnitronitrosoguanidine (MNNG) was used to produce a CAG model in Wistar rats, and HE staining was used to determine the pathological model. LC-MS was used to detect the differential metabolic profiles in rat urine. Diversified analysis was performed by the statistical method. Results Compared with the control group, the model group had 68 differential metabolites, 25 that were upregulated and 43 that were downregulated. The main metabolic pathways were D-glutamine and D-glutamic acid metabolism, histidine metabolism and purine metabolism. Conclusion By searching for differential metabolites and metabolic pathways in the urine of CAG rats, this study provides effective experimental data for the pathogenesis and clinical diagnosis of CAG.