Open AccessPhotoprotective effect of Astragalus membranaceus polysaccharide on UVA-induced damage in HaCaT cells
Author(s) -
Qiong Li,
Depeng Wang,
Donghui Bai,
Chao Cai,
Jia Li,
Chengxiu Yan,
Shuai Zhang,
Zhijun Wu,
Jiejie Hao,
Guangli Yu
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0235515
Subject(s) - hacat , polysaccharide , intracellular , oxidative stress , astragalus , oxidative damage , chemistry , mitochondrion , microbiology and biotechnology , biochemistry , biology , in vitro , medicine , alternative medicine , traditional chinese medicine , pathology
Background The skin provides a predominant barrier against chemical, physical and microbial incursion. The intemperate exposure to ultraviolet A (UVA) radiation can cause excessive cellular oxidative stress, leading to skin damage, proteins damage and mitochondrial dysfunction. There is sufficient evidences supporting the proposal that mitochondria is highly implicated in skin photo-damage. Methods In the present study, a polysaccharide isolated from Astragalus membranaceus was further purified to be an α-glucan, which was further investigated its beneficial influence on UVA-induced photo-damage in HaCaT cells. Results Our results showed that the purified Astragalus membranaceus polysaccharide (AP) can protect HaCaT cells from UVA-induced photo-damage through reducing UVA-induced intracellular ROS production and mitochondrial membrane potential, thereby altering ATP content. It was found that the UVA induced damage in HaCaT cells could be effectively restored by co-treatment with AP. Conclusions AP exhibited promising potential for advanced application as multifunctional skin care products and drugs.