Open AccessNew Andean source of resistance to anthracnose and angular leaf spot: Fine-mapping of disease-resistance genes in California Dark Red Kidney common bean cultivar
Author(s) -
Maria Celeste GonçalvesVidigal,
Thiago Alexandre Santana Gílio,
Giseli Valentini,
Mariana Vaz-Bisneta,
Pedro Soares Vidigal Filho,
Qijian Song,
Paula Rodrigues Oblessuc,
Maeli Melotto
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0235215
Subject(s) - biology , colletotrichum lindemuthianum , population , genetics , leaf spot , plant disease resistance , quantitative trait locus , cultivar , gene , botany , demography , sociology
Anthracnose (ANT) and angular leaf spot (ALS) caused by Colletotrichum lindemuthianum and Pseudocercospora griseola , respectively, are devastating diseases of common bean around the world. Therefore, breeders are constantly searching for new genes with broad-spectrum resistance against ANT and ALS. This study aimed to characterize the genetic resistance of California Dark Red Kidney (CDRK) to C . lindemuthianum races 73, 2047, and 3481 and P . griseola race 63–39 through inheritance, allelism testing, and molecular analyses. Genetic analysis of response to ANT and ALS in recombinant inbred lines (RILs) from a CDRK × Yolano cross (CY) showed that the resistance of CDRK cultivar is conferred by a single dominant loci, which we named CoPv01 CDRK /PhgPv01 CDRK . Allelism tests performed with race 3481showed that the resistance gene in CDRK is independent of the Co-1 and Co-AC . We conducted co-segregation analysis in genotypes of 110 CY RILs and phenotypes of the RILs in response to different races of the ANT and ALS pathogens. The results revealed that CoPv01 CDRK and PhgPv01 CDRK are coinherited, conferring resistance to all races. Genetic mapping of the CY population placed the CoPv01 CDRK / PhgPv01 CDRK loci in a 245 Kb genomic region at the end of Pv01. By genotyping 19 RILs from the CY population using three additional markers, we fine-mapped the CoPv01 CDRK / PhgPv01 CDRK loci to a smaller genomic region of 33 Kb. This 33 Kb region harbors five predicted genes based on the common bean reference genome. These results can be applied in breeding programs to develop bean cultivars with ANT and ALS resistance using marker-assisted selection.