Open AccessPesticide-tolerant bacteria isolated from a biopurification system to remove commonly used pesticides to protect water resources
Author(s) -
Gabriela Briceño,
Claudio Lamilla,
Bárbara Leiva,
Marcela Levio,
Pamela Donoso-Piñol,
Heidi Schalchli,
Felipe Gallardo,
M.C. Díez
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0234865
Subject(s) - achromobacter , chlorpyrifos , microbiology and biotechnology , bacteria , pesticide , pseudomonas , biology , metabolite , actinobacteria , food science , proteobacteria , chemistry , 16s ribosomal rna , biochemistry , ecology , genetics
In this study, we selected and characterized different pesticide-tolerant bacteria isolated from a biomixture of a biopurification system that had received continuous applications of a pesticides mixture. The amplicon analysis of biomixture reported that the phyla Proteobacteria, Firmicutes, Bacteroidetes and Actinobacteria were predominant. Six strains grew in the presence of chlorpyrifos and iprodione. Biochemical characterization showed that all isolates were positive for esterase, acid phosphatase, among others, and they were identified as Pseudomonas , Rhodococcus and Achromobacter based on molecular and proteomic analysis. Bacterial growth decreased as both pesticide concentrations increased from 10 to 100 mg L -1 in liquid culture. The Achromobacter sp. strain C1 showed the best chlorpyrifos removal rate of 0.072–0.147 d -1 a half-life of 4.7–9.7 d and a maximum metabolite concentration of 2.10 mg L -1 at 120 h. On the other hand, Pseudomonas sp. strain C9 showed the highest iprodione removal rate of 0.100–0.193 d -1 a half-life of 4–7 d and maximum metabolite concentration of 0.95 mg L -1 at 48 h. The Achromobacter and Pseudomonas strains showed a good potential as chlorpyrifos and iprodione-degrading bacteria.