Open AccessThe profile of lipid metabolites in urine of marmoset wasting syndrome
Author(s) -
Arisa Yamazaki,
Tatsuro Nakamura,
Takako MiyabeNishiwaki,
Akihiro Hirata,
Rikako Inoue,
Koji Kobayashi,
Yusuke Miyazaki,
Yuta Hamasaki,
Akiyo Ishigami,
Nanae Nagata,
Akihisa Kaneko,
Makoto Koizumi,
Hiroyuki Ohta,
Hirotaka James Okano,
Takahisa Murata
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0234634
Subject(s) - marmoset , callithrix , urine , arachidonic acid , endocrinology , eicosapentaenoic acid , medicine , urinary system , metabolite , eicosanoid , polyunsaturated fatty acid , chemistry , biology , biochemistry , fatty acid , paleontology , enzyme
Marmoset wasting syndrome (MWS) is clinically characterized by progressive weight loss. Although morbidity and mortality of MWS are relatively high in captive marmosets, its causes remain unknown. Lipid mediators are bioactive metabolites which are produced from polyunsaturated fatty acids, such as arachidonic acid (AA) and eicosapentaenoic acid. These lipid metabolites regulate a wide range of inflammatory responses and they are excreted into the urine. As urinary lipid profiles reflect systemic inflammatory conditions, we comprehensively measured the levels of 141 types of lipid metabolites in the urines obtained from healthy common marmoset ( Callithrix jacchus ) (N = 7) or marmosets with MWS (N = 7). We found that 41 types of metabolites were detected in all urine samples of both groups. Among them, AA-derived metabolites accounted for 63% (26/41 types) of all detected metabolites. Notably, the levels of AA-derived prostaglandin (PG) E 2 , PGF 2α , thromboxane (TX) B 2 and F 2 -isoprostanes significantly increased in the urine samples of marmosets with MWS. In this study, we found some urinary lipid metabolites which may be involved in the development of MWS. Although the cause of MWS remains unclear, our findings may provide some insight into understanding the mechanisms of development of MWS.