Open AccessAdhesion of streptococci to titanium and zirconia
Author(s) -
Yukinobu Oda,
Tadashi Miura,
Gentaro Mori,
Hodaka Sasaki,
Taichi Ito,
Masao Yoshinari,
Yasutomo Yajima
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0234524
Subject(s) - streptococcus sanguinis , streptococcus gordonii , streptococcus oralis , streptococcus mutans , materials science , x ray photoelectron spectroscopy , cubic zirconia , adhesion , contact angle , scanning electron microscope , titanium , wetting , surface roughness , biofilm , microbiology and biotechnology , nuclear chemistry , chemistry , composite material , bacteria , chemical engineering , metallurgy , biology , ceramic , engineering , genetics
The purpose of this study was to evaluate the adherence of streptococci to disks of titanium (commercially pure titanium: CpTi) and zirconia (tetragonal zirconia polycrystals: TZP). CpTi and yttria-stabilized TZP disks with a mirror-polished surface were used as specimens. The arithmetic mean surface roughness (Ra and Sa) and the surface wettability of the experimental specimens were measured. For analyzing the outermost layer of the experimental specimens, X-ray photoelectron spectroscopy (XPS) analysis was performed. Streptococcus sanguinis , S . gordonii , S . oralis , and S . mutans were used as streptococcal bacterial strains. These bacterial cultures were grown for 24 h on CpTi and TZP. The number of bacterial adhesions was estimated using an ATP-bioluminescent assay, and scanning electron microscope (SEM) observation of the adhered bacterial specimens was performed. No significant differences in surface roughness or wettability were found between CpTi and TZP. In XPS analyses, outermost layer of CpTi included Ti0 and Ti 4+ , and outermost layer of TZP included Zr 4+ . In the cell adhesion assay, the adherences of S . sanguinis , S . gordonii , and S . oralis to TZP were significantly lower than those to CpTi (p < 0.05); however, significant difference was not observed for S . mutans among the specimens. The adherence to CpTi and TZP of S . mutans was significantly lower than that of S . sanguinis , S . gordonii , and S . oralis . These results were confirmed by SEM. S . sanguinis , S . gordonii , and S . oralis adhered less to TZP than to CpTi, but the adherence of S . mutans was similar to both surfaces. S . mutans was less adherent compare with the other streptococci tested in those specimens.