Open AccessProteomics of intracellular freezing survival
Author(s) -
Michael A. S. Thorne,
Nina Kočevar Britovšek,
Liam J. Hawkins,
Kathryn S. Lilley,
Kenneth B. Storey
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0233048
Subject(s) - proteome , intracellular , trehalose , biology , downregulation and upregulation , proteomics , dehydration , microbiology and biotechnology , acclimatization , nematode , freezing tolerance , biochemistry , ecology , gene
Panagrolaimus sp. DAW1, a nematode cultured from the Antarctic, has the extraordinary physiological ability to survive total intracellular freezing throughout all of its compartments. While a few other organisms, all nematodes, have subsequently also been found to survive freezing in this manner, P . sp. DAW1 has so far shown the highest survival rates. In addition, P . sp. DAW1 is also, depending on the rate or extent of freezing, able to undergo cryoprotective dehydration. In this study, the proteome of P . sp DAW1 is explored, highlighting a number of differentially expressed proteins and pathways that occur when the nematodes undergo intracellular freezing. Among the strongest signals after being frozen is an upregulation of proteases and the downregulation of cytoskeletal and antioxidant activity, the latter possibly accumulated before freezing much in the way the sugar trehalose has been shown to be stored during acclimation.