Open AccessApplication of co-culture technology of epithelial type cells and mesenchymal type cells using nanopatterned structures
Author(s) -
Taek-Hee Jung,
Eun-Bin Chung,
Hyung Woo Kim,
Seong Woo Choi,
SoonJung Park,
Anthony Safaa Mukhtar,
Hyung-Min Chung,
EunMi Kim,
Kang Moo Huh,
Dong Sung Kim,
Sun-Woong Kang,
Sung-Hwan Moon
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0232899
Subject(s) - mesenchymal stem cell , microbiology and biotechnology , nanopillar , cell culture , cell type , cell adhesion , cell , cell growth , induced pluripotent stem cell , cellular differentiation , chemistry , materials science , nanotechnology , biology , embryonic stem cell , biochemistry , nanostructure , genetics , gene
Various nanopatterning techniques have been developed to improve cell proliferation and differentiation efficiency. As we previously reported, nanopillars and pores are able to sustain human pluripotent stem cells and differentiate pancreatic cells. From this, the nanoscale patterns would be effective environment for the co-culturing of epithelial and mesenchymal cell types. Interestingly, the nanopatterning selectively reduced the proliferative rate of mesenchymal cells while increasing the expression of adhesion protein in epithelial type cells. Additionally, co-cultured cells on the nanopatterning were not negatively affected in terms of cell function metabolic ability or cell survival. This is in contrast to conventional co-culturing methods such as ultraviolet or chemical treatments. The nanopatterning appears to be an effective environment for mesenchymal co-cultures with typically low proliferative rates cells such as astrocytes, neurons, melanocytes, and fibroblasts without using potentially damaging treatments.