Open AccessFunctional cycle of EEA1-positive early endosome: Direct evidence for pre-existing compartment of degradative pathway
Author(s) -
Rimma Kamentseva,
Vera V. Kosheverova,
M. V. Kharchenko,
Maria Zlobina,
А.В. Салова,
T. N. Belyaeva,
Nikolay Nikolsky,
Е. С. Корнилова
Publication year - 2020
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0232532
Subject(s) - endosome , endocytic cycle , microbiology and biotechnology , vesicle , endocytosis , biogenesis , biology , chemistry , biochemistry , receptor , membrane , intracellular , gene
Early endosomes, regarded as the main sorting station on endocytic pathway, are characterized by high frequency of homotypic fusions mediated by tethering protein EEA1. Despite intensive investigations, biogenesis of endosomes, boundaries between early and late endosomes, and process of cargo transition though them remain obscure. Here, using EGF/EGFR endocytosis as a model and confocal microscopy of fixed and live cells, we provide evidence favoring EEA1-vesicles being pre-existed vesicular compartment, that maintains its resident proteins’ level and is sensitive to biosynthetic, but not endocytic pathway disturbance. EEA1-vesicles directly fuse with incoming EGF/EGFR-vesicles into hybrid endosomes with separated EEA1- and EGFR-domains, thus providing a platform for rapid achievement of an excess of surface-derived membrane that is used to form intraluminal vesicles (ILVs). Thus, multivesicular structures colocalized with EEA1 are still early endosomes. “EEA1-cycle” ends by exclusion of EGFR-containing domains with ILVs inside that turns into MVE and restoration of initial EEA1-vesicles population.